Stahlberg, HenningDubochet, JacquesVogel, HorstGhosh, Robin2006-02-272006-02-272006-02-27199810.1006/jmbi.1998.1975https://infoscience.epfl.ch/handle/20.500.14299/226329WOS:000076192300011The basic photosynthetic unit contg. the reaction center and the light-harvesting I complex (RC-LHI) of the purple non-sulfur bacterium Rhodospirillum rubrum was purified and reconstituted into two-dimensional (2D) membrane crystals. Transmission electron microscopy using conventional techniques and cryoelectron microscopy of the purified single particles and of 2D crystals yielded a projection of the RC-LHI complex at a resoln. of at least 1.6 nm. In this projection the LHI ring appears to have a square symmetry and packs in a square crystal lattice. The square geometry of the LHI ring was obsd. also in images of single isolated particles of the RC-LHI complex. However, although the LHI units are packed identically within the crystal lattice, a new rotational anal. developed here showed that the reaction centers take up one of four possible orientations within the ring. This fourfold disorder supports our interpretation of a square ring symmetry and suggests that a hitherto undetected component may be present within the photosynthetic unit. (c) 1998 Academic Press. [on SciFinder (R)]text::journal::journal article::research article