Linciano, SaraMoro, GiuliaZorzi, AlessandroAngelini, Alessandro2022-07-182022-07-182022-07-182022-08-0110.1016/j.jconrel.2022.05.038https://infoscience.epfl.ch/handle/20.500.14299/189337WOS:000815942500002Human serum albumin (hSA) is the major carrier protein for fatty acids (FAs) in plasma. Its ability to bind multiple FA moieties with moderate to high affinity has inspired the use of FA conjugation as a safe and natural platform to generate long-lasting therapeutics with enhanced pharmacokinetic properties and superior efficacy. In this frame, the choice of the FA is crucial and a comprehensive elucidation of the molecular interactions of FAs with hSA cannot be left out of consideration. To this intent, we report here a comparative analysis of the binding mode of different FA moieties with hSA. The choice among different albumin-binding FAs and how this influence the pharmacokinetics properties of a broad spectrum of therapeutic molecules will be discussed including a critical description of some clinically relevant FA conjugated therapeutics.Chemistry, MultidisciplinaryPharmacology & PharmacyChemistryserum albuminalbumin bindingfatty acidlipidationlipidated therapeuticpost-translational chemical modificationbioconjugationdrug deliverypharmacokinetichalf-lifeglucagon-like peptide-1human glp-1 analogbinding sitesinsulin detemiraffinityidentificationoptimizationsemaglutidetext::journal::journal article::review article