Bulushev, Roman D.Marion, SanjinPetrova, EkaterinaDavis, Sebastian J.Maerkl, Sebastian J.Radenovic, Aleksandra2016-12-072016-12-072016-12-07201610.1021/acs.nanolett.6b04165https://infoscience.epfl.ch/handle/20.500.14299/131841WOS:000389963200084Through the use of optical tweezers we performed controlled translocations of DNA-protein complexes through nanocapillaries. We used RNA polymerase (RNAP) with two binding sites on a 7.2 kbp DNA fragment and a dCas9 protein tailored to have five binding sites on lambda-DNA (48.5 kbp). Measured localization of binding sites showed a shift from the expected positions on the DNA that we explained using both analytical fitting and a stochastic model. From the measured force versus stage curves we extracted the non equilibrium work done during the translocation of a DNA-protein complex and used it to obtain an estimate of the effective charge of the complex. In combination with conductivity measurements, we provided a proof of concept for discrimination between different DNA protein complexes simultaneous to the localization of their binding sites.Nanoporenanocapillaryforce measurementsDNA-protein complexprotein binding siteJarzynski equationtext::journal::journal article::research article