Lorthe, ElsaBellon, MathildeMichielin, GregoireBerthelot, JulieZaballa, Maria-EugeniaPennacchio, FrancescoBekliz, MeriemLaubscher, FlorianArefi, FatemehPerez-Saez, JavierAzman, Andrew S.L'Huillier, Arnaud G.Posfay-Barbe, Klara M.Kaiser, LaurentGuessous, IdrisMaerkl, Sebastian J.Eckerle, IsabellaStringhini, Silvia2022-09-122022-09-122022-09-122022-08-1710.1371/journal.pone.0272663https://infoscience.epfl.ch/handle/20.500.14299/190734WOS:000847227000054ObjectivesTo report a prospective epidemiological, virological and serological investigation of a SARS-CoV-2 outbreak in a primary school.MethodsAs part of a longitudinal, prospective, school-based surveillance study, this investigation involved repeated testing of 73 pupils, 9 teachers, 13 non-teaching staff and 26 household members of participants who tested positive, with rapid antigen tests and/or RT-PCR (Day 0-2 and Day 5-7), serologies on dried capillary blood samples (Day 0-2 and Day 30), contact tracing interviews and SARS-CoV-2 whole genome sequencing.ResultsWe identified 20 children (aged 4 to 6 years from 4 school classes), 2 teachers and a total of 4 household members who were infected by the Alpha variant during this outbreak. Infection attack rates were between 11.8 and 62.0% among pupils from the 4 school classes, 22.2% among teachers and 0% among non-teaching staff. Secondary attack rate among household members was 15.4%. Symptoms were reported by 63% of infected children, 100% of teachers and 50% of household members. All analysed sequences but one showed 100% identity. Serological tests detected 8 seroconversions unidentified by SARS-CoV-2 virological tests.ConclusionsThis study confirmed child-to-child and child-to-adult SARS-CoV-2 transmission and introduction into households. Effective measures to limit transmission in schools have the potential to reduce the overall community circulation.Multidisciplinary SciencesScience & Technology - Other Topicsa h1n1 virushousehold transmissionelementary-schoolcountytext::journal::journal article::research article