Generosi, JohannaMargaritondo, GiorgioKropf, MichelHirling, HaraldCatsicas, StefanJohnsson, KaiTolk, Norman H.Piston, David W.Cricenti, Antonio2009-03-122009-03-122009-03-12200810.1063/1.3003567https://infoscience.epfl.ch/handle/20.500.14299/35926WOS:000262605800166High-resolution detection of specific molecules in cells is a major challenge in biology. We show that infrared scanning near-field microscopy can detect the spatial distribution of alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid–type glutamate receptor clusters on hippocampal neurons. The GluR2 subunits were labeled with the die Alexa 488 and high-resolution infrared micrographs were taken at 6.25 µm. The absence of photobleaching makes this approach suitable for a long-term observation and allows to localize different infrared-absorbing molecules over the complex background of other cell components.text::journal::journal article::research article