Gassner, Anne-LaureProczek, GaelleGirault, Hubert H.2011-12-162011-12-162011-12-16201110.1007/s00216-011-5417-1https://infoscience.epfl.ch/handle/20.500.14299/73279WOS:000297159900019A bubble cell capillary classically used to extend the optical path length for UV-vis detection is employed here to trap magnetic beads. With this system, a large amount of beads can be captured without inducing a strong pressure drop, as it is the case with magnetic beads trapped in a standard capillary, thereby having less effect on the experimental conditions. Using numerical simulations and microscopic visualizations, the capture of beads inside a bubble cell was investigated with two magnet configurations. Pressure-driven and electro-osmotic flow velocities were measured for different amounts of protein-A-coated beads or C18-functionalized beads (RPC-18). Solid-phase extraction of a model antibody on protein-A beads and preconcentration of fluorescein on RPC-18 beads were performed as proof of concept experiments.Magnetic particleBubble cellSolid-phase extractionNumerical simulationFinite element methodSolid-Phase ExtractionSpe-CeChipImmunoassayParticlesProteinLineSeparationMatricesPolymertext::journal::journal article::research article