Gas cluster ion beam SEM for imaging of large tissue samples with 10 nm isotropic resolution

Hayworth, Kenneth J.Peale, DavidJanuszewski, MichalKnott, Graham W.Lu, ZhiyuanXu, C. ShanHess, Harald F.2020-03-032020-03-032020-03-032020-11-1810.1038/s41592-019-0641-2https://infoscience.epfl.ch/handle/20.500.14299/166771WOS:000508582900037We demonstrate gas cluster ion beam scanning electron microscopy (SEM), in which wide-area ion milling is performed on a series of thick tissue sections. This three-dimensional electron microscopy technique acquires datasets with <10 nm isotropic resolution of each section, and these can then be stitched together to span the sectioned volume. Incorporating gas cluster ion beam SEM into existing single-beam and multibeam SEM workflows should be straightforward, increasing reliability while improving z resolution by a factor of three or more.Biochemical Research MethodsBiochemistry & Molecular BiologyplasticityGas cluster ion beam SEM for imaging of large tissue samples with 10 nm isotropic resolutiontext::journal::journal article::research article