Alfaro, Javier AntonioBohländer, PeggyDai, MingjieFilius, MikeHoward, Cecil J.van Kooten, Xander F.Ohayon, ShiloPomorski, AdamSchmid, SonjaAksimentiev, AlekseiAnslyn, Eric V.Bedran, GeorgesCao, ChanChinappi, MauroCoyaud, EtienneDekker, CeesDittmar, GunnarDrachman, NicholasEelkema, RienkGoodlett, DavidHentz, SébastienKalathiya, UmeshKelleher, Neil L.Kelly, Ryan T.Kelman, ZviKim, Sung HyunKuster, BernhardRodriguez-Larrea, DavidLindsay, StuartMaglia, GiovanniMarcotte, Edward M.Marino, John P.Masselon, ChristopheMayer, MichaelSamaras, PatroklosSarthak, KumarSepiashvili, LusiaStein, DerekWanunu, MeniWilhelm, MathiasYin, PengMeller, AmitJoo, Chirlmin2021-06-072021-06-072021-06-072021-06-0710.1038/s41592-021-01143-1https://infoscience.epfl.ch/handle/20.500.14299/178706Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics.text::journal::journal article::review article