Pereira, Sarah A. P.Baptista, A. Catarina L.Biancalana, LorenzoMarchetti, FabioDyson, Paul J.Saraiva, M. Lucia M. F. S.2022-06-202022-06-202022-06-202022-12-3110.1080/14756366.2022.2073443https://infoscience.epfl.ch/handle/20.500.14299/188611WOS:000802813800001A novel automated method based on sequential injection analysis (SIA), a non-segmented flow injection technique, was developed to evaluate glutathione S-transferase P1-1 (GST P1-1) activity in the presence of organometallic complexes with putative anticancer activity. The assay is based on the reaction of L-glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB) in the presence of GST P1-1 to afford the GS-DNB conjugate and the reaction may be monitored by an increase in absorbance at 340 nm. A series of ruthenium, iron, osmium and iridium complexes were evaluated as GST P1-1 inhibitors by evaluating their half-maximal inhibitory concentration (IC50). An iridium compound displays the lowest IC50 value of 6.7 +/- 0.7 mu M and an iron compound displays the highest IC50 value of 275 +/- 9 mu M. The SIA method is simple to use, robust, reliable, and efficient and uses fewer reagents than batch methods and each analysis takes only 5 minutes.Biochemistry & Molecular BiologyChemistry, MedicinalPharmacology & Pharmacysequential injection analysis (sia)glutathione s-transferase p1-1enzyme inhibition assaysanticancer metal complexesruthenium arene complexesin-vitroenzymedrugmechanismagentstext::journal::journal article::research article