Gambarotto, DavideZwettler, Fabian U.Le Guennec, MaevaSchmidt-Cernohorska, MarketaFortun, DenisBorgers, SusanneHeine, JoernSchloetel, Jan-GeroReuss, MatthiasUnser, MichaelBoyden, Edward S.Sauer, MarkusHamel, VirginieGuichard, Paul2019-01-232019-01-232019-01-232019-01-0110.1038/s41592-018-0238-1https://infoscience.epfl.ch/handle/20.500.14299/153970WOS:000454162400032Determining the structure and composition of macromolecular assemblies is a major challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an extension of expansion microscopy that allows the visualization of preserved ultrastructures by optical microscopy. This method allows for near-native expansion of diverse structures in vitro and in cells; when combined with super-resolution microscopy, it unveiled details of ultrastructural organization, such as centriolar chirality, that could otherwise be observed only by electron microscopy.Biochemical Research MethodsBiochemistry & Molecular Biologypolyglutamylated tubulinlocalizationproteinsrevealstext::journal::journal article::research article