Charrière, FlorianMarian, AncaColomb, TristanMarquet, PierreDepeursinge, Christian2009-07-202009-07-202009-07-20200710.1364/OL.32.002456https://infoscience.epfl.ch/handle/20.500.14299/41628WOS:00024932760006211117We present here a three-dimensional evaluation of the amplitude point-spread function (APSF) of a microscope objective (MO), based on a single holographic acquisition of its pupil wavefront. The aberration function is extracted from this pupil measurements and then inserted in a scalar model of diffraction, allowing one to calculate the distribution of the complex wavefront propagated around the focal point. The accuracy of the results is compared with a direct measurement of the APSF with a second holographic system located in the image plane of the MO. Measurements on a 100× 1.3 NA MO are presented.[MVD]HolographyAberration compensationComputer holographyImaging systemsAperturesOptical transfer functionstext::journal::journal article::research article