Liu, XiaohaiMilton, JohnSmith, GeoffreyBarnes, ColinRasolonjatovo, IsabelleRigatti, RobertoWu, XiaolinOst, TobiasWorsley, GrahamEarnshaw, DavidTurcatti, GerardoRomieu, Anthony2008-05-132008-05-132008-05-132009https://infoscience.epfl.ch/handle/20.500.14299/23731The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5' end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reactionpatentES2398808EP2298930US10710046US2018353926US9999866US2017021325US9498763US2015343410US9085802US2014349891US8715966ES2398808EP2298930US2012208194EP1910559HK1154636EP2298930US2009118128EP1910559WO2007010251WO2007010251GB051493634897551