Chen, ZhiChen, WenshengLu, Hsin-YuChevallier, YvesChen, NanguangBarbastathis, GeorgeLuo, Yuan2014-08-292014-08-292014-08-29201410.1364/Ol.39.003078https://infoscience.epfl.ch/handle/20.500.14299/106541WOS:000336982500066Holographic optical tweezers (HOTs) extend optical trapping into three dimensions. Volume imaging then becomes a concern as trapped objects are easily moved out of focus of the imaging objective lens. Here we demonstrate a three-dimensional real-time interactive optical trapping, manipulating, and imaging system based on HOTs incorporated with volume holographic microscope. Intensity information about the trapped objects at multiple depths can be captured in a single measurement. This method is compatible with most imaging modes such as bright-field and fluorescence. (C) 2014 Optical Society of Americatext::journal::journal article::research article