Buban, James P.Ramasse, QuentinGipson, BryantBrowning, Nigel D.Stahlberg, Henning2020-02-132020-02-132020-02-132009-11-1410.1093/jmicro/dfp052https://infoscience.epfl.ch/handle/20.500.14299/165442During the past two decades instrumentation in scanning transmission electron microscopy (STEM) has pushed toward higher intensity electron probes to increase the signal-to-noise ratio of recorded images. While this is suitable for robust specimens, biological specimens require a much reduced electron dose for high-resolution imaging. We describe here protocols for low-dose STEM image recording with a conventional field-emission gun STEM, while maintaining the high-resolution capability of the instrument. Our findings show that a combination of reduced pixel dwell time and reduced gun current can achieve radiation doses comparable to low-dose TEM.text::journal::journal article::research article