Wurm, FrederikSteinbach, TobiasKlok, Harm-Anton2013-10-012013-10-012013-10-01201310.1039/c3cc44039ghttps://infoscience.epfl.ch/handle/20.500.14299/95881WOS:000322915200017A water-soluble squaric acid dialkyl diester derivative is presented, which enables one-pot, two-step amine-selective protein conjugation reactions with (functional) amines in water. This reagent not only allows all-aqueous protein modifications, but also tolerates e. g. hydroxyl groups and can also be used for the modification of proteins with water-insoluble amines.text::journal::journal article::research article