Delattre, M.Leidel, S.Wani, K.Baumer, K.Bamat, J.Schnabel, H.Feichtinger, R.Schnabel, R.Gönczy, P.2006-08-242006-08-242006-08-24200410.1038/ncb1146https://infoscience.epfl.ch/handle/20.500.14299/23378815232593Centrosomes, the major microtubule-organizing centres (MTOCs) of animal cells, are comprised of a pair of centrioles surrounded by pericentriolar material (PCM). Early in the cell cycle, there is a single centrosome, which duplicates during S-phase to direct bipolar spindle assembly during mitosis. Although crucial for proper cell division, the mechanisms that govern centrosome duplication are not fully understood. Here, we identify the Caenorhabditis elegans gene sas-5 as essential for daughter-centriole formation. SAS-5 is a coiled-coil protein that localizes primarily to centrioles. Fluorescence recovery after photobleaching (FRAP) experiments with green fluorescent protein (GFP) fused to SAS-5 (GFP-SAS-5) demonstrated that the protein shuttles between centrioles and the cytoplasm throughout the cell cycle. Analysis of mutant alleles revealed that the presence of SAS-5 at centrioles is crucial for daughter-centriole formation and that ZYG-1, a kinase that is also essential for this process, controls the distribution of SAS-5 to centrioles. Furthermore, partial RNA-interference (RNAi)-mediated inactivation experiments suggest that both sas-5 and zyg-1 are dose-dependent regulators of centrosome duplication.Active TransportCell Nucleus/geneticsAnimalsCaenorhabditis elegans/genetics/*metabolism/ultrastructureCaenorhabditis elegans Proteins/genetics/isolation &purification/*metabolismCell Cycle Proteins/genetics/isolation & purification/*metabolismCentrioles/*metabolism/ultrastructureCentrosome/*metabolism/ultrastructureGene DosageMicroscopyElectronMitosis/*geneticsMolecular Sequence DataProtein Kinases/genetics/metabolismProtein Transport/geneticsRNA Interference/physiologyResearch SupportNon-U.S. Gov'tResearch SupportU.S. Gov'tP.H.S.text::journal::journal article::research article