Braschler, ThomasValero, AnaColella, LudovicaPataky, KristopherBrugger, JürgenRenaud, Philippe2010-11-022010-11-022010-11-02201010.1039/c004988chttps://infoscience.epfl.ch/handle/20.500.14299/56555WOS:000282314200018Controlling alginate gel formation by diffusion of Ca2+ ions through a filter barrier, a layer-by-layer deposition technique with resolution on the size scale of a single cell is presented. It offers the possibility of exposing cells under biocompatible conditions to microheterogeneous three-dimensional environments, mimicking the layered structure of extracellular matrix in tissues.Fluorescein DiacetatePhotolithographyDifferentiationViabilitySurfacestext::journal::journal article::research article