Wurm, F.Bernard, A.2007-06-052007-06-052007-06-05199910.1016/S0958-1669(99)80027-5https://infoscience.epfl.ch/handle/20.500.14299/7633WOS:00007952350000910209142Large-scale transient expression from mammalian cells is a new technology. Breakthroughs have been achieved for non-viral delivery methods: transfections can now be done at the 1-10 L scale with mammalian cells grown in suspension. Production of 1-20 mg/L of recombinant protein have been obtained in stirred bioreactors. Modified alphaviruses have provided a fast and efficient expression technology based on viral vectors.Adenoviridae/geneticsAlphavirus/geneticsAnimalsCell Culture TechniquesCell LineCercopithecus aethiopsCloningMolecularCricetinaeGenetic VectorsHumansRecombinant Proteins/*biosynthesis/geneticsVaccinia virus/geneticstext::journal::journal article::research article