Ramdya, Pavan P.Schaffter, ThomasFloreano, DarioBenton, Richard2012-09-282012-09-282012-09-28201210.1371/journal.pone.0048381https://infoscience.epfl.ch/handle/20.500.14299/85781WOS:000311935800050Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discriminate subpopulations, imaging hardware that simultaneously records behavior and fluorescence expression, and open-source software for automated, high-accuracy determination of genetic identity. Using FBI, we measure courtship partner choice in genetically mixed groups of Drosophila.DrosophilaBehaviorFluorescenceMicroscopeImage AnalysisFBIsQuidEvolutionary Roboticstext::journal::journal article::research article