Detecting single molecules launched for liquid surfaces in a time-of-flight mass spectrometer using ultraviolet and infrared lasers

Twerenbold, D.Netuschill, A.de Rooij, N. F.Luginbuhl, Ph.Gerber, D.Gritti, D.Gonin, Y.Rossel, F.Vuilleumier, J.-L.2009-05-122009-05-122009-05-12200210.1002/1615-9861(200204)2:4<436::AID-PROT436>3.0.CO;2-Ihttps://infoscience.epfl.ch/handle/20.500.14299/39960The launch of molecules from liquid surfaces in a time of flight mass spectrometer has been investigated using different sample preparation techniques, and by exposing the liquid samples to two different laser wavelengths, 337 nm from a N2 ultraviolet laser and 10.6 μm from a CO2 infrared laser. The molecules were detected with cryodetectors measuring the energy of the individual molecules. We present insulin and lysozyme results from samples introduced into the vacuum through a micromachined silicon injector, and from samples consisting of a glycerol droplet deposited directly on the sample holder at the high voltage stage of the ion optics.Detecting single molecules launched for liquid surfaces in a time-of-flight mass spectrometer using ultraviolet and infrared laserstext::journal::journal article::research article