Liu, YZhong, WMeng, SKong, JLu, HYang, PGirault, HHLiu, B2006-10-052006-10-052006-10-05200610.1002/chem.200501622https://infoscience.epfl.ch/handle/20.500.14299/235003WOS:000240358200014A biocompatible interface was constructed on a microchip by using the layer-by-layer (LBL) assembly of charged polysaccharides incorporating proteases for highly efficient proteolysis. The controlled assembly of natural polyelectrolytes and the enzyme-adsorption step were monitored by using a quartz-crystal microbalance and atomic force microscopy (AFM). Such a multilayer-assembled membrane provides a biocompatible interconnected network with high enzyme-loading capacity. The maximum digestion rate of the adsorbed trypsin in a microchannel was significantly accelerated to 1600 mM min-interfaceslayer-by-layer assemblymicrochip reactorsproteomicstrypsintext::journal::journal article::research article