Application of an asymmetric flow field flow fractionation (AF4) multi-detector approach for metallic ENP characterization - prospects and limitations demonstrated on Au nanoparticles

Hagendorfer, H.Kaegi, R.Traber, J.Mertens, S. F.Scherrers, R.Ludwig, C.Ulrich, A.2011-08-102011-08-102011-08-10201110.1016/j.aca.2011.08.014https://infoscience.epfl.ch/handle/20.500.14299/69960WOS:000297184600023In this work we discuss about the method development, applicability and limitations of an asymmetric flow field flow fractionation (A4F) system in combination with a multi-detector setup consisting of UV/vis, light scattering, and inductively coupled plasma mass spectrometry (ICPMS). The overall aim was to obtain a size dependent-, element specific-, and quantitative method appropriate for the characterization of metallic engineered nanoparticle (ENP) dispersions. Thus, systematic investigations of crucial method parameters were performed by employing well characterized Au nanoparticles (Au-NPs) as a defined model system.Asymmetric flow field flow fractionationLight scatteringIcpmsMetallic engineered nanoparticlesAu nanoparticlesParticle-membrane interactionPlasma-Mass SpectrometrySizeColloidsNanomaterialsCytotoxicityEnvironmentParticlesReleaseFacadesIcpmsApplication of an asymmetric flow field flow fractionation (AF4) multi-detector approach for metallic ENP characterization - prospects and limitations demonstrated on Au nanoparticlestext::journal::journal article::research article