Cradick, Thomas J.Ambrosini, GiovannaIseli, ChristianBucher, PhilippMcCaffrey, Anton P.2011-12-162011-12-162011-12-16201110.1186/1471-2105-12-152https://infoscience.epfl.ch/handle/20.500.14299/73970WOS:000291659600002Background: Zinc Finger Nucleases (ZFNs) are man-made restriction enzymes useful for manipulating genomes by cleaving target DNA sequences. ZFNs allow therapeutic gene correction or creation of genetically modified model organisms. ZFN specificity is not absolute; therefore, it is essential to select ZFN target sites without similar genomic off-target sites. It is important to assay for off-target cleavage events at sites similar to the target sequence.Artificial Transcription FactorsDouble-Strand BreaksDna-SequencesMajor DeterminantDomainsRecognitionFamilyCellsConstructionDimerizationtext::journal::journal article::research article