Brun, Matthias A.Tan, Kui-ThongGriss, RudolfKielkowska, AnnaReymond, LucJohnsson, Kai2012-06-082012-06-082012-06-08201210.1021/ja3002277https://infoscience.epfl.ch/handle/20.500.14299/81426WOS:000303696200026We report the semisynthesis of a fluorescent glutamate sensor protein on cell surfaces. Sensor excitation at 547 nm yields a glutamate-dependent emission spectrum between 550 and 700 nm that can be exploited for ratiometric sensing. On cells, the sensor displays a ratiometric change of 1.56. The high sensitivity toward glutamate concentration changes of the sensor and its exclusive extracellular localization make it an attractive tool for glutamate sensing in neurobiology.ReceptorAgonisttext::journal::journal article::research article