Kenzaoui, Blanka HalamodaBernasconi, Catherine ChapuisHofmann, HeinrichJuillerat-Jeanneret, Lucienne2012-03-152012-03-152012-03-15201210.2217/NNM.11.85https://infoscience.epfl.ch/handle/20.500.14299/78798WOS:000300212600015Aim: Ultrasmall superparamagnetic iron oxide nanoparticles (USPIO-NPs) are under development for imaging and drug delivery; however, their interaction with human blood brain barrier models is not known. Materials & Methods: The uptake, reactive oxygen species production and transport of USPIO-NPs across human brain-derived endothelial cells as models of the blood brain tumor barrier were evaluated for either uncoated, oleic acid-coated or polyvinylamine-coated USPIO-NPs. Results: Reactive oxygen species production was observed for oleic acid-coated and polyvinylamine-coated USPIO-NPs. The uptake and intracellular localization of the iron oxide core of the USPIO-NPs was confirmed by transmission electron microscopy. However, while the uptake of these USPIO-NPs by cells was observed, they were neither released by nor transported across these cells even in the presence of an external dynamic magnetic field. Conclusion: USPIO-NP-loaded filopodia were observed to invade the polyester membrane, suggesting that they can be transported by migrating angiogenic brain-derived endothelial cells.cell uptakeelectron microscopyhuman brain-derived endothelial cellsiron oxide nanoparticlesmagnetic enhancementoxidative stresstranscellular transportPoly(Butyl Cyanoacrylate) NanoparticlesDrug-DeliveryPlga NanoparticlesOxidative StressIn-VitroBarrierDoxorubicinToxicityGlioblastomaPermeabilitytext::journal::journal article::research article