Sivagnanam, VenkataragavaluSong, BoVandevyver, CarolineBünzli, Jean-Claude G.Gijs, Martin A. M.2010-06-172010-06-17201010.1021/la9045572https://infoscience.epfl.ch/handle/20.500.14299/50894WOS:000276969700004Separation and subsequent culturing of MCF-7 breast cancer cells on self-assembled protein-coated magnetic beads in a microfluidic chip is demonstrated. The beads were patterned in situ inside a sealed microfluidic channel using magnetic-field-assisted electrostatic self-assembly. Hereafter, they were grafted by exposure to a solution of 5D10 monoclonal antibodies (mAb) and fibronectin (FN), with the first being used for immunospecific cell capture and the latter being used for cell adhesion and growth. A solution of target MCF-7 cells mixed with Jurkat cells was brought inside the microchannel, leading to specific MCF-7 cell capture; the latter were then cultured and evidenced by cell immuno-luminescence.Total Analysis SystemsMammalian-CellsDeviceAdhesionLysisFibronectinSeparationProteinDepositionLabeltext::journal::journal article::research article