Hassler, K.Rigler, P.Blom, H.Rigler, R.Widengren, J.Lasser, T.2008-02-252008-02-252008-02-25200710.1364/OE.15.005366https://infoscience.epfl.ch/handle/20.500.14299/19108WOS:00024639500001110584This paper discusses the application of objective-type total internal reflection fluorescence correlation spectroscopy (TIR-FCS) to the study of the kinetics of immobilized horseradish peroxidase on a single molecule level. Objective-type TIR-FCS combines the advantages of FCS with TIRF microscopy in a way that allows for simultaneous ultra-sensitive spectroscopic measurements using a single-point detector and convenient localization of single molecules on a surface by means of parallel imaging.(170.2520) Medical optics and biotechnologyFluorescence microscopy(300.2530) SpectroscopyFluorescencelaser-induced(300.6490) SpectroscopySpectroscopysurfacetext::journal::journal article::research article