Hannebelle, Melanie T. M.Raeth, EstherLeitao, Samuel M.Lukes, TomasPospisil, JakubToniolo, ChiaraVenzin, Olivier FrançoisChrisnandy, AntoniusSwain, Prabhu PrasadRonceray, NathanLutolf, Matthias POates, Andrew C.Hagen, Guy M.Lasser, TheoRadenovic, AleksandraMcKinney, John DFantner, Georg E2024-04-172024-04-172024-04-172024-02-2010.1038/s41467-024-45567-7https://infoscience.epfl.ch/handle/20.500.14299/207162WOS:001176075200034Super-resolution techniques expand the abilities of researchers who have the knowledge and resources to either build or purchase a system. This excludes the part of the research community without these capabilities. Here we introduce the openSIM add-on to upgrade existing optical microscopes to Structured Illumination super-resolution Microscopes (SIM). The openSIM is an open-hardware system, designed and documented to be easily duplicated by other laboratories, making super-resolution modality accessible to facilitate innovative research. The add-on approach gives a performance improvement for pre-existing lab equipment without the need to build a completely new system.|Researchers developed an open-hardware structured illumination microscopy add-on. This affordable upgrade provides super-resolution capabilities for normal optical microscopes. Detailed instructions enable easy reproduction to help democratize advanced microscopy.Superresolution MicroscopyResolution LimitLive CellsLighttext::journal::journal article::research article