Leidel, S.Gönczy, P.2006-08-242006-08-242006-08-24200310.1016/S1534-5807(03)00062-5https://infoscience.epfl.ch/handle/20.500.14299/23378512636923The mechanisms governing centrosome duplication remain poorly understood. We identified a gene called sas-4 that is essential for this process in C. elegans. SAS-4 encodes a predicted coiled-coil protein that localizes to a tiny dot in the center of centrosomes throughout the cell cycle. FRAP experiments with GFP-SAS-4 transgenic embryos reveal that SAS-4 is recruited to the centrosome once per cell cycle, at the time of organelle duplication. Additional evidence indicates that SAS-4 is recruited to the daughter centriole or a closely associated structure. These findings identify SAS-4 recruitment as a key step in the centrosome duplication cycle.AnimalsCaenorhabditis elegans/cytology/*genetics/*metabolismCaenorhabditis elegans Proteins/*genetics/*metabolismCell Cycle/*geneticsCentrioles/genetics/metabolismCentrosome/*metabolismEmbryoNonmammalian/cytology/metabolismFemaleFluorescence Resonance Energy TransferFluorescent Antibody TechniqueGene Expression Regulation/geneticsGerm Cells/cytology/metabolismGreen Fluorescent ProteinsLuminescent Proteins/diagnostic useMaleMolecular Sequence DataProtein Kinases/deficiency/geneticsRNA Interference/physiologyResearch SupportNon-U.S. Gov'tSpermatozoa/cytology/metabolismtext::journal::journal article::research article