A DMSO-sensitive conditional mutant of the fission yeast orthologue of the Saccharomyces cerevisiae SEC13 gene is defective in septation

Poloni, D.Simanis, V.2008-10-212008-10-212008-10-21200210.1016/S0014-5793(01)03285-9https://infoscience.epfl.ch/handle/20.500.14299/30360Dissection of complex processes using model organisms such as yeasts relies heavily upon the use of conditional mutants. We have generated a collection of fission yeast mutants sensitive to dimethylsulphoxide (DMSO). Among these we have found a mutant in the Schizosaccharomyces pombe orthologue of the Saccharomyces cerevisiae SEC13 gene, which fails to cleave the division septum. Generation of a null allele demonstrates that the S. pombe sec13 gene is essential.Amino Acid SequenceCell Division/drug effects/geneticsCell Survival/geneticsCloningMolecularDimethyl Sulfoxide/*pharmacologyGenesEssential/geneticsMembrane Proteins/chemistry/genetics/*metabolismMolecular Sequence DataMutagenesis/geneticsMutation/drug effects/*geneticsNuclear Pore Complex ProteinsSaccharomyces cerevisiae/*geneticsSaccharomyces cerevisiae ProteinsSchizosaccharomyces/*cytology/drug effects/*genetics/metabolismSchizosaccharomyces pombe Proteins/chemistry/genetics/metabolismTime FactorsA DMSO-sensitive conditional mutant of the fission yeast orthologue of the Saccharomyces cerevisiae SEC13 gene is defective in septationtext::journal::journal article::research article