Heinis, ChristianBertschinger, JulianNeri, Dario2008-11-172008-11-172008-11-172003https://infoscience.epfl.ch/handle/20.500.14299/31197The procedures for calmodulin-tagged phage and two-filter sandwich assays for the isolation of enzymic activities are presented. In the first method, enzymes are displayed on filamentous phage, and the reaction substrate is anchored on the calmodulin-tagged phage-enzymes using a calmodulin binding peptide. In the second method, enzyme mutants are expressed in bacterial colonies on a porous "master" filter, and the enzymes are released and diffuse to a second reaction filter that closely contacts the master filter. [on SciFinder (R)]BacteriophageFilamentous bacteriophage (calmodulin-tagged phage and two-filter sandwich assays for identification of enzyme activities)Enzymes Role: ANT (Analyte)BSU (Biological study unclassified)ANST (Analytical study)BIOL (Biological study) (calmodulin-tagged phage and two-filter sandwich assays for identification of enzyme activities)Calmodulins Role: ARU (Analytical role unclassified)BUU (Biological use unclassified)ANST (Analytical study)BIOL (Biological study)USES (Uses) (calmodulin-tagged phage and two-filter sandwich assays for identification of enzyme activities)enzyme activity calmodulin tagged phage two filter sandwich assaytext::book/monograph::book part or chapter