Skowicki, MichalHurlimann, DimitriTarvirdipour, ShabnamKyropoulou, MyrtoSchoenenberger, Cora-AnnGerber-Lemaire, SandrinePalivan, Cornelia G.2024-02-232024-02-232024-02-232024-01-2410.1021/acs.biomac.3c00943https://infoscience.epfl.ch/handle/20.500.14299/205556WOS:001161575800001As current chemo- and photodynamic cancer therapies are associated with severe side effects due to a lack of specificity and to systemic toxicity, innovative solutions in terms of targeting and controlled functionality are in high demand. Here, we present the development of a polymersome nanocarrier equipped with targeting molecules and loaded with photosensitizers for efficient uptake and light-activated cell killing. Polymersomes were self-assembled in the presence of photosensitizers from a mixture of nonfunctionalized and functionalized PDMS-b-PMOXA diblock copolymers, the latter designed for coupling with targeting ligands. By encapsulation inside the polymersomes, the photosensitizer Rose Bengal was protected, and its uptake into cells was mediated by the nanocarrier. Inhibitor of fibroblast activation protein alpha (FAPi), a ligand for FAP, was attached to the polymersomes' surface and improved their uptake in MCF-7 breast cancer cells expressing relatively high levels of FAP on their surface. Once internalized by MCF-7, irradiation of Rose Bengal-loaded FAPi-polymersomes generated reactive oxygen species at levels high enough to induce cell death. By combining photosensitizer encapsulation and specific targeting, polymersomes represent ideal candidates as therapeutic nanocarriers in cancer treatment.Life Sciences & BiomedicinePhysical SciencesPhotodynamic TherapyCancerDeliveryNanoparticlesNanoreactorsStrategiesLiposomesProteintext::journal::journal article::research article