Innovative, Non-stirred Bioreactors in Scales from Milliliters up to 1000 Liters for Suspension Cultures of Cells using Disposable Bags and Containers

Werner, SörenEibl, RegineLettenbauer, ChristineRöll, MarcelEibl, DieterDe Jesus, MariaZhang, XiaoweiStettler, MatthieuTissot, StephanieBürkie, CedricBroccard, GillesKühner, MarkusTanner, RolfBaldi, LuciaHacker, DavidWurm, Florian M.2011-02-112011-02-112011-02-11201010.2533/chimia.2010.819https://infoscience.epfl.ch/handle/20.500.14299/64223WOS:000285277300012Innovative mixing principles in bioreactors, for example using the rocking of a platform to induce a backwards and forwards 'wave' , or using orbital shaking to generate a 'wave' that runs round in a cylindrical container, have proved to be successful for the suspension cultures of cells, especially when combined with disposable materials. This article presents an overview of the engineering characteristics when these new principles are applied in bioreactors, and case studies covering scales of operation from milliliters to 1000 liters.Disposable bioreactorsEngineering characteristicsHigh density CHO culturesOrbitally shakenWave-mixedAnimal-CellsCultivationPlantTechnologyDeviceInnovative, Non-stirred Bioreactors in Scales from Milliliters up to 1000 Liters for Suspension Cultures of Cells using Disposable Bags and Containers – A Swiss Contributiontext::journal::journal article::research article