Infection of mice with aerosolized Mycobacterium tuberculosis: use of a nose-only apparatus for delivery of low doses of inocula and design of an ultrasafe facility

Schwebach, J ReidChen, BingGlatman-Freedman, AharonaCasadevall, ArturoMcKinney, John DHarb, John LMcGuire, Patrick JBarkley, W EmmettBloom, Barry RJacobs, William R2010-09-072010-09-072010-09-07200210.1128/AEM.68.9.4646-4649.2002https://infoscience.epfl.ch/handle/20.500.14299/5283012200325Aerosolized delivery of virulent or hypervirulent Mycobacterium tuberculosis requires careful consideration of methodology and safety. To maximize safety, we installed a nose-only aerosol apparatus that can reproducibly deliver a low dose (<100 CFU per mouse) of M. tuberculosis in a carefully designed biohazard facility.Mycobacterium tuberculosisInfection of mice with aerosolized Mycobacterium tuberculosis: use of a nose-only apparatus for delivery of low doses of inocula and design of an ultrasafe facilitytext::journal::journal article::research article