000098670 001__ 98670
000098670 005__ 20190316233910.0
000098670 037__ $$aARTICLE
000098670 245__ $$aMetabolic engineering under uncertainty-II: Analysis of yeast metabolism
000098670 269__ $$a2006
000098670 260__ $$c2006
000098670 336__ $$aJournal Articles
000098670 500__ $$a16-8 FIELD Section Title:Fermentation and Bioindustrial Chemistry
000098670 500__ $$a10, 48
000098670 500__ $$aDepartment of Chemical and Biological Engineering,Northwestern University,Evanston,IL,USA. FIELD URL:
000098670 500__ $$awritten in English.
000098670 520__ $$aYeast metab. has been used extensively in scientific investigations and industrial applications. Understanding the properties of the yeast metabolic network is crucial, yet unaccomplished due to its high complexity, the different culture conditions, and the uncertainty assocd. with kinetic parameters. We recently developed a computational and math. framework using Monte Carlo method in which parameter uncertainty can be addressed through large-scale sampling procedure. This framework was applied on the compartmentalized central carbon pathways of Saccharomyces cerevisiae metab. considering the growth environment of batch and chemostat reactor and integrating information from metabolic flux anal. Statistical anal. of the results indicates that yeast cells growing in batch culture condition exhibit dramatically different control schemes from those growing in a chemostat. The difference is mainly due to the feedback introduced by the constraints of the chemostat. The control of the enzymes on the rates of the substrate uptake, product excretion, and cell growth and its practical implication are discussed. Clustering of the reaction steps according to the similarity of their responses to enzyme activity perturbations reveals functional coupling of metabolic reactions. [on SciFinder (R)]
000098670 6531_ $$aSaccharomyces metab flux analysis
000098670 700__ $$aWang, Liqing
000098670 700__ $$g174688$$aHatzimanikatis, Vassily$$0240657
000098670 773__ $$j8$$tMetabolic Engineering$$k2$$q142-159
000098670 8564_ $$uhttps://infoscience.epfl.ch/record/98670/files/vh-2006-3.pdf$$zn/a$$s875614
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000098670 937__ $$aLCSB-ARTICLE-2006-004
000098670 970__ $$a0000000001/LCSB
000098670 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000098670 980__ $$aARTICLE