Inactivation of Notch1 impairs VDJbeta rearrangement and allows pre-TCR-independent survival of early alpha beta Lineage Thymocytes

Notch proteins influence cell fate decisions in many developmental systems. During lymphoid development, Notch1 signaling is essential to direct a bipotent T/B precursor toward the T cell fate, but the role of Notch1 at later stages of T cell development remains controversial. We have recently reported that tissue-specific inactivation of Notch1 in immature (CD44(-) CD25(+)) thymocytes does not affect subsequent T cell development. Here, we demonstrate that loss of Notch1 signaling at an earlier (CD44(+)CD25(+)) developmental stage results in severe perturbation of alpha beta but not gamma delta lineage development. Immature Notch1(-/-) thymocytes show impaired VDJ beta rearrangement and aberrant pre-TCR-independent survival. Collectively, our data demonstrate that Notch1 controls several nonredundant functions necessary for alpha beta lineage development.


Published in:
Immunity, 16, 6, 869-79
Year:
2002
Keywords:
Note:
Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, 1066, Epalinges, Switzerland.
1074-7613 (Print)
Journal Article
Laboratories:




 Record created 2006-12-05, last modified 2018-03-17

External link:
Download fulltext
URL
Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)