Peptide and protein characterization by high-rate electron capture dissociation Fourier transform ion cyclotron resonance mass spectrometry
A review. The anal. utility of the electron capture dissocn. (ECD) technique, developed by McLafferty and co-workers, has substantially improved peptide and protein characterization using Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS). The limitations of the first ECD implementations on com. instruments were eliminated by the employment of low-energy electron-injection systems based on indirectly heated dispenser cathodes. In particular, the ECD rate and reliability were greatly increased, enabling the combination of ECD/FTICR-MS with online liq. sepn. techniques. Further technique development allowed the combination of two rapid fragmentation techniques, high-rate ECD and IR multiphoton dissocn. (IRMPD), in a single exptl. configuration. Simultaneous and consecutive irradiations of trapped ions with electrons and photons extended the possibilities for ion activation/dissocn. and led to improved peptide and protein characterization. The application of high-rate ECD/FTICR-MS has demonstrated its power and unique capabilities in top-down sequencing of peptides and proteins, including characterization of post-translational modifications, improved sequencing of peptides with multiple disulfide bridges and secondary fragmentation (w-ion formation). Anal. of peptide mixts. has been accomplished using high-rate ECD in bottom-up mass spectrometry based on mixt. sepn. by liq. chromatog. and capillary electrophoresis. This paper summarizes the current impact of high-rate ECD/FTICR-MS for top-down and bottom-up mass spectrometry of peptides and proteins. [on SciFinder (R)]
34-0 FIELD Section Title:Amino Acids, Peptides, and Proteins
Division of Ion Physics, .ANG.ngstroem Laboratory,Uppsala University,Uppsala,Swed. FIELD URL:
written in English.
Record created on 2006-11-09, modified on 2016-08-08