Antioxidant Redox Sensors Based on DNA Modified Carbon Screen-Printed Electrodes

Antioxidant redox sensors based on DNA modified carbon screen-printed electrodes were developed. The carbon ink was doped with TiO2 nanoparticles, onto which double- strand DNA was adsorbed. A redox mediator, namely, tris- 2,2′-bipyridine ruthenium(II) [Ru(bpy)32+] was electro- oxidized on the electrode surface to subsequently oxidize both the adsorbed ds-DNA and the antioxidants in solu- tion. The resulting oxidation damage of the adsorbed ds- DNA was then detected by square wave voltammetry in a second solution containing only Ru(bpy)3Cl2 at a low con- centration (μM). A kinetic model was developed to study the protecting role of antioxidants in aqueous solutions. The electrochemical sensor has been applied to evaluate the redox antioxidant capacity of different molecules.


Published in:
Analytical Chemistry, 78, 6879-6884
Year:
2006
Other identifiers:
DAR: 9333
Laboratories:




 Record created 2006-11-02, last modified 2018-03-17

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