Assembly-Controlled Biocompatible Interface on a Microchip: Strategy to Highly Efficient Proteolysis

A biocompatible interface was constructed on a microchip by using the layer-by-layer (LBL) assembly of charged polysaccharides incorporating proteases for highly efficient proteolysis. The controlled assembly of natural polyelectrolytes and the enzyme-adsorption step were monitored by using a quartz-crystal microbalance and atomic force microscopy (AFM). Such a multilayer-assembled membrane provides a biocompatible interconnected network with high enzyme-loading capacity. The maximum digestion rate of the adsorbed trypsin in a microchannel was significantly accelerated to 1600 mM min-


Published in:
Chemistry - A European Journal, 12, 6585-6591
Year:
2006
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 Record created 2006-10-05, last modified 2018-03-17

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