Parallel single molecule detection with a fully integrated single-photon 2X2 CMOS detector array

We present parallel single molecule detection (SMD) and fluorescence correlation spectroscopy (FCS) experiments with a fully integrated complementary metal oxide semiconductor (CMOS) single-photon 2 X 2 detector array. Multifocal excitation is achieved with a diffractive optical element (DOE). Special emphasis is placed on parallelization of the total system. The performance of the novel single-photon CMOS detector is investigated and compared to a state-of-the-art single-photon detecting module [having an actively quenched avalanche photodiode (APD)] by measurements on free diffusing molecules at different concentrations. Despite the order of magnitude lower detection efficiency of the CMOS detector compared to the state-of-the-art single-photon detecting module, we achieve single molecule sensitivity and reliably determine molecule concentrations. In addition, the CMOS detector performance for the determination of the fraction of slowly diffusing molecules in a primer solution (two-component analysis) is demonstrated. The potential of this new technique for high-throughput confocal-detection-based systems is discussed. (C) 2004 Society of Photo-Optical Instrumentation Engineers.

Published in:
Journal of Biomedical Optics, 9, 5, 913-921
Karolinska Inst, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden. Ecole Polytech Fed Lausanne, Lab Opt Biomed, CH-1015 Lausanne, Switzerland. Ecole Polytech Fed Lausanne, Inst Microelect & Microsyst, CH-1015 Lausanne, Switzerland. Royal Inst Technol, Dept Microelect & Informat Technol, S-16440 Kista, Sweden. Karolinska Inst, Dept Med Biochem & Biophys, SE-17177 Stockholm, Sweden. Rigler, R, Karolinska Inst, Dept Med Biochem & Biophys, SE-17177 Stockholm, Sweden.
ISI Document Delivery No.: 860PY
Times Cited: 6
Cited Reference Count: 29

 Record created 2006-10-03, last modified 2018-03-17

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