Immunohistochemistry for the MEPHISTO X-PEEM
Over almost 50 years of its development, the science of immunology has become an indispensable tool for the understanding of the histology of tissues. Antibodies are highly specific probes, so that tissue structure can be interpreted not just by morphological considerations but by association with a wide variety of physiological molecular antigens. The simple concept of an antibody linked to a microscopically dense marker remains constant in each application of the technique, such as the use of fluorescent markers or the incorporation of electron-dense gold colloids for electron microscopy. We describe a new application of immunocytochemistry to x-ray spectromicroscopy: the antibody labeling of tissue structures with nickel precipitates. Regions of positive staining can be seen by the acquisition of nickel distribution maps in the MEPHISTO X-PEEM from the intense Ni L-edge absorption features around 850 eV. The aim of this work is to know the background tissue structures on which the distribution of other relevant elements (such as boron for BNCT) can be mapped. Results are presented showing positive staining by two antibodies in human glioblastoma tissue, anti-Ki-67, a protein found in the nuclei of proliferating cells, and anti-van Willebrandt factor, located in blood vessel endothelia. We show that the criteria for successful staining for optical microscopy are different than for spectroscopic imaging, but useful results can be obtained with careful image treatment.
Swiss Fed Inst Lausanne, Inst Appl Phys, Lausanne, Switzerland.Gilbert, B, Swiss Fed Inst Lausanne, Inst Appl Phys, Lausanne, Switzerland.
ISI Document Delivery No.: BX39K
Cited Reference Count: 7
6th International Conference on X-Ray Microscopy
AUG 02-06, 1999
2 HUNTINGTON QUADRANGLE, STE 1NO1, MELVILLE, NY 11747-4501 USA
Record created on 2006-10-03, modified on 2016-08-08