Cell ashing for trace element analysis: A new approach based on ultraviolet/ozone
1999
Abstract
We studied a new approach to cell ashing based on illuminating the specimens with a low-pressure mercury discharge lamp. We analyzed with synchrotron spectromicroscopy its effects on different physiological elements in neurobiological specimens. Our results demonstrate that carbon is removed, whereas phosphorus, calcium, potassium, and sulfur are retained and their relative concentrations are enhanced. Applied to trace elements, this technique will enhance their practical detectability. (C) 1999 Academic Press.
Details
Title
Cell ashing for trace element analysis: A new approach based on ultraviolet/ozone
Author(s)
De Stasio, G. ; Gilbert, B. ; Perfetti, L. ; Hansen, R. ; Mercanti, D. ; Ciotti, M. T. ; Andres, R. ; White, V. E. ; Perfetti, P. ; Margaritondo, G.
Published in
Analytical Biochemistry
Volume
266
Issue
2
Pages
174-180
Date
1999
ISSN
0003-2697
Keywords
Note
CNR, Ist Struttura Mat, I-00133 Rome, Italy. Ecole Polytech Fed Lausanne, Inst Phys Appl, PH Ecublens, CH-1015 Lausanne, Switzerland. Univ Roma Tor Vergata, Rome, Italy. Univ Wisconsin, Ctr Synchrotron Radiat, Stoughton, WI 53589 USA. CNR, Ist Neurobiol, I-00137 Rome, Italy. Paul Scherrer Inst, CH-5232 Villigen, Switzerland. CALTECH, Jet Prop Lab, Pasadena, CA 91109 USA. Sincrotrone Trieste SCpA, Trieste, Italy. De Stasio, G, CNR, Ist Struttura Mat, Via Fosso Cavaliere, I-00133 Rome, Italy.
ISI Document Delivery No.: 162LY
ISI Document Delivery No.: 162LY
Other identifier(s)
View record in Web of Science
Record Appears in
Scientific production and competences > SB - School of Basic Sciences > SB Archives > LSE - Laboratory of Photoelectron Spectroscopy
Scientific production and competences > SB - School of Basic Sciences > SB Archives > LPRX - X-Ray Physics Laboratory
Peer-reviewed publications
Work produced at EPFL
Journal Articles
Published
Scientific production and competences > SB - School of Basic Sciences > SB Archives > LPRX - X-Ray Physics Laboratory
Peer-reviewed publications
Work produced at EPFL
Journal Articles
Published
Record creation date
2006-10-03