Cell ashing for trace element analysis: A new approach based on ultraviolet/ozone

We studied a new approach to cell ashing based on illuminating the specimens with a low-pressure mercury discharge lamp. We analyzed with synchrotron spectromicroscopy its effects on different physiological elements in neurobiological specimens. Our results demonstrate that carbon is removed, whereas phosphorus, calcium, potassium, and sulfur are retained and their relative concentrations are enhanced. Applied to trace elements, this technique will enhance their practical detectability. (C) 1999 Academic Press.


Published in:
Analytical Biochemistry, 266, 2, 174-180
Year:
1999
ISSN:
0003-2697
Keywords:
Note:
CNR, Ist Struttura Mat, I-00133 Rome, Italy. Ecole Polytech Fed Lausanne, Inst Phys Appl, PH Ecublens, CH-1015 Lausanne, Switzerland. Univ Roma Tor Vergata, Rome, Italy. Univ Wisconsin, Ctr Synchrotron Radiat, Stoughton, WI 53589 USA. CNR, Ist Neurobiol, I-00137 Rome, Italy. Paul Scherrer Inst, CH-5232 Villigen, Switzerland. CALTECH, Jet Prop Lab, Pasadena, CA 91109 USA. Sincrotrone Trieste SCpA, Trieste, Italy. De Stasio, G, CNR, Ist Struttura Mat, Via Fosso Cavaliere, I-00133 Rome, Italy.
ISI Document Delivery No.: 162LY
Laboratories:




 Record created 2006-10-03, last modified 2018-01-27


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