Native and modified uncoated neurons observed by atomic force microscopy
Dried, fixed uncoated neuron granule cells and their neurites have been imaged in air by an atomic force microscope (AFM) in the repulsive regime of contact mode. Neurons have also been observed after swelling with glutamate or decapping. Modifications induced by glutamate resulted in a drastic reduction in the cell body height (from 1.5 to 2.0 mu m for the untreated ones down to 0.7-1.0 mu m) and in an increased corrugation of the granule cell membrane. In the decapped neurons AFM revealed decreasing cell body height for an increasing amount of removed material during the decapping procedure. These results demonstrate that AFM is the ideal technique to observe cell corrugation and height modifications during cell treatments of neurobiological interest. (C) 1996 American Vacuum Society.
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Ecole polytech fed lausanne,inst phys appl,ch-1015 lausanne,switzerland. cnr,ist neurobiol,i-00100 rome,italy. cnr,ist biol cellulare,i-00100 rome,italy. Cricenti, A, CNR,IST STRUTTURA MAT,VIA ENRICO FERMI 38,I-00044 FRASCATI,ITALY.
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Record created on 2006-10-03, modified on 2016-08-08