Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans
A key challenge of functional genomics today is to generate well-annotated data sets that can be interpreted across different platforms and technologies. Large-scale functional genomics data often fail to connect to standard experimental approaches of gene characterization in individual laboratories. Furthermore, a lack of universal annotation standards for phenotypic data sets makes it difficult to compare different screening approaches. Here we address this problem in a screen designed to identify all genes required for the first two rounds of cell division in the Caenorhabditis elegans embryo. We used RNA-mediated interference to target 98% of all genes predicted in the C. elegans genome in combination with differential interference contrast time-lapse microscopy. Through systematic annotation of the resulting movies, we developed a phenotypic profiling system, which shows high correlation with cellular processes and biochemical pathways, thus enabling us to predict new functions for previously uncharacterized genes.
- URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15791247
Keywords: Animals ; Caenorhabditis elegans/*embryology/*genetics/physiology ; Caenorhabditis elegans Proteins/*genetics/*metabolism ; Computational Biology ; Embryonic Development/*genetics ; Genes ; Helminth/genetics ; *Genome ; Genomics ; Phenotype ; *RNA Interference ; RNA ; Helminth/genetics/metabolism ; RNA ; Messenger/genetics/metabolism ; Research Support ; Non-U.S. Gov't
Cenix BioScience GmbH, Tatzberg 47-51, D-01307 Dresden, Germany. email@example.com 1476-4687 (Electronic) Journal Article
Record created on 2006-08-24, modified on 2016-08-08