000089338 001__ 89338
000089338 005__ 20180317093009.0
000089338 0247_ $$2doi$$a10.1016/S1534-5807(03)00062-5
000089338 037__ $$aARTICLE
000089338 245__ $$aSAS-4 is essential for centrosome duplication in C elegans and is recruited to daughter centrioles once per cell cycle
000089338 269__ $$a2003
000089338 260__ $$c2003
000089338 336__ $$aJournal Articles
000089338 500__ $$aSwiss Institute for Experimental, Cancer Research (ISREC), CH-1066 Epalinges/, Lausanne, Switzerland.
000089338 500__ $$a1534-5807 (Print)
000089338 500__ $$aJournal Article
000089338 520__ $$aThe mechanisms governing centrosome duplication remain poorly understood. We identified a gene called sas-4 that is essential for this process in C. elegans. SAS-4 encodes a predicted coiled-coil protein that localizes to a tiny dot in the center of centrosomes throughout the cell cycle. FRAP experiments with GFP-SAS-4 transgenic embryos reveal that SAS-4 is recruited to the centrosome once per cell cycle, at the time of organelle duplication. Additional evidence indicates that SAS-4 is recruited to the daughter centriole or a closely associated structure. These findings identify SAS-4 recruitment as a key step in the centrosome duplication cycle.
000089338 6531_ $$aAnimals
000089338 6531_ $$aCaenorhabditis elegans/cytology/*genetics/*metabolism
000089338 6531_ $$aCaenorhabditis elegans Proteins/*genetics/*metabolism
000089338 6531_ $$aCell Cycle/*genetics
000089338 6531_ $$aCentrioles/genetics/metabolism
000089338 6531_ $$aCentrosome/*metabolism
000089338 6531_ $$aEmbryo
000089338 6531_ $$aNonmammalian/cytology/metabolism
000089338 6531_ $$aFemale
000089338 6531_ $$aFluorescence Resonance Energy Transfer
000089338 6531_ $$aFluorescent Antibody Technique
000089338 6531_ $$aGene Expression Regulation/genetics
000089338 6531_ $$aGerm Cells/cytology/metabolism
000089338 6531_ $$aGreen Fluorescent Proteins
000089338 6531_ $$aLuminescent Proteins/diagnostic use
000089338 6531_ $$aMale
000089338 6531_ $$aMolecular Sequence Data
000089338 6531_ $$aProtein Kinases/deficiency/genetics
000089338 6531_ $$aRNA Interference/physiology
000089338 6531_ $$aResearch Support
000089338 6531_ $$aNon-U.S. Gov't
000089338 6531_ $$aSpermatozoa/cytology/metabolism
000089338 700__ $$aLeidel, S.
000089338 700__ $$0243625$$aGönczy, P.$$g168676
000089338 773__ $$j4$$k3$$q431-9$$tDev Cell
000089338 8564_ $$uhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12636923$$zURL
000089338 909CO $$ooai:infoscience.tind.io:89338$$particle$$pSV
000089338 909C0 $$0252248$$pUPGON$$xU11155
000089338 937__ $$aUPGON-ARTICLE-2003-005
000089338 970__ $$a16/UPGON
000089338 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000089338 980__ $$aARTICLE