SAS-4 is essential for centrosome duplication in C elegans and is recruited to daughter centrioles once per cell cycle
The mechanisms governing centrosome duplication remain poorly understood. We identified a gene called sas-4 that is essential for this process in C. elegans. SAS-4 encodes a predicted coiled-coil protein that localizes to a tiny dot in the center of centrosomes throughout the cell cycle. FRAP experiments with GFP-SAS-4 transgenic embryos reveal that SAS-4 is recruited to the centrosome once per cell cycle, at the time of organelle duplication. Additional evidence indicates that SAS-4 is recruited to the daughter centriole or a closely associated structure. These findings identify SAS-4 recruitment as a key step in the centrosome duplication cycle.
- URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12636923
Keywords: Animals ; Caenorhabditis elegans/cytology/*genetics/*metabolism ; Caenorhabditis elegans Proteins/*genetics/*metabolism ; Cell Cycle/*genetics ; Centrioles/genetics/metabolism ; Centrosome/*metabolism ; Embryo ; Nonmammalian/cytology/metabolism ; Female ; Fluorescence Resonance Energy Transfer ; Fluorescent Antibody Technique ; Gene Expression Regulation/genetics ; Germ Cells/cytology/metabolism ; Green Fluorescent Proteins ; Luminescent Proteins/diagnostic use ; Male ; Molecular Sequence Data ; Protein Kinases/deficiency/genetics ; RNA Interference/physiology ; Research Support ; Non-U.S. Gov't ; Spermatozoa/cytology/metabolism
Swiss Institute for Experimental, Cancer Research (ISREC), CH-1066 Epalinges/, Lausanne, Switzerland.
Record created on 2006-08-24, modified on 2016-08-08