000089312 001__ 89312
000089312 005__ 20181203020532.0
000089312 037__ $$aARTICLE
000089312 245__ $$aA single amino acid can determine the DNA binding specificity of homeodomain proteins
000089312 269__ $$a1989
000089312 260__ $$c1989
000089312 336__ $$aJournal Articles
000089312 500__ $$aHoward Hughes Medical Institute, Rockefeller University, New York, New York 10021-6399.
000089312 500__ $$a0092-8674 (Print)
000089312 500__ $$aJournal Article
000089312 520__ $$aMany Drosophila developmental genes contain a DNA binding domain encoded by the homeobox. This homeodomain contains a region distantly homologous to the helix-turn-helix motif present in several prokaryotic DNA binding proteins. We investigated the nature of homeodomain-DNA interactions by making a series of mutations in the helix-turn-helix motif of the Drosophila homeodomain protein Paired (Prd). This protein does not recognize sequences bound by the homeodomain proteins Fushi tarazu (Ftz) or Bicoid (Bcd). We show that changing a single amino acid at the C-terminus of the recognition helix is both necessary and sufficient to confer the DNA binding specificity of either Ftz or Bcd on Prd. This simple rule indicates that the amino acids that determine the specificity of homeodomains are different from those mediating protein-DNA contacts in prokaryotic proteins. We further show that Prd contains two DNA binding activities. The Prd homeodomain is responsible for one of them while the other is not dependent on the recognition helix.
000089312 6531_ $$aAmino Acid Sequence
000089312 6531_ $$aAnimals
000089312 6531_ $$aBase Sequence
000089312 6531_ $$aDNA-Binding Proteins/*genetics
000089312 6531_ $$aDeoxyribonuclease I
000089312 6531_ $$aDrosophila/*genetics
000089312 6531_ $$a*Genes
000089312 6531_ $$aHomeobox
000089312 6531_ $$aMolecular Sequence Data
000089312 6531_ $$aMutation
000089312 6531_ $$aNucleotide Mapping
000089312 6531_ $$aOligonucleotide Probes
000089312 6531_ $$aPlasmids
000089312 6531_ $$aProtein Conformation
000089312 6531_ $$aResearch Support
000089312 6531_ $$aNon-U.S. Gov't
000089312 700__ $$aTreisman, J.
000089312 700__ $$0243625$$aGönczy, P.$$g168676
000089312 700__ $$aVashishtha, M.
000089312 700__ $$aHarris, E.
000089312 700__ $$aDesplan, C.
000089312 773__ $$j59$$k3$$q553-62$$tCell
000089312 8564_ $$uhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2572327$$zURL
000089312 909C0 $$0252248$$pUPGON$$xU11155
000089312 909CO $$ooai:infoscience.tind.io:89312$$pSV$$particle
000089312 937__ $$aUPGON-ARTICLE-1989-002
000089312 970__ $$a38/UPGON
000089312 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000089312 980__ $$aARTICLE