Mapping the binding sites of peptide and non-peptide molecules to G protein-coupled receptors by fluorescence

Novel fluorescence approaches to investigate ligand recognition and structure of G protein-coupled receptors in native membranes have been developed. These methods combine the biosynthetic incorporation of unnatural fluorescent amino acids at known sites in receptors with the technique of fluorescence energy transfer for distance measurement. This permits one to fix the ligand in space and to define the structure of the receptor in a model of ligand-receptor interactions. Subdomains of ligand binding sites on NK1 and NK2 receptors were also characterized using environment-sensitive fluorophores and the techniques of collisional quenching and anisotropy. Antagonists and agonists have different binding sites on NK1 and NK2.


Publié dans:
Letters in Peptide Science, 5, 2-3, 79-82
Année
1998
Mots-clefs:
Laboratoires:




 Notice créée le 2006-08-14, modifiée le 2018-12-03


Évaluer ce document:

Rate this document:
1
2
3
 
(Pas encore évalué)