000088450 001__ 88450
000088450 005__ 20181203020500.0
000088450 0247_ $$2doi$$a10.1016/0022-1759(92)90253-P
000088450 037__ $$aARTICLE
000088450 245__ $$aPurification and characterization of biologically active human recombinant 37 kDa soluble CD23 (sFc epsilon RII) expressed in insect cells
000088450 269__ $$a1992
000088450 260__ $$c1992
000088450 336__ $$aJournal Articles
000088450 520__ $$aHuman recombinant soluble 37 kDa CD23 has been expressed in insect cells and secreted into the culture medium using the IL-2 leader sequence. The 37 kDa CD23 was purified 600-fold to homogeneity by monoclonal antibody affinity chromatography and gel filtration. The pure protein is monomeric, glycosylated, depleted of one N terminal amino acid and contains four disulphide bonds. It degrades into smaller fragments of 33, 29 and 25 kDa if purified in the absence of protease inhibitors. The same pattern of proteolytic fragments is observed when the pure preparation is incubated at room temperature for 3 weeks. Physical characterization of the 37 kDa CD23 by circular dichroism indicates that the protein contains mainly beta sheet and 20% of alpha helical structures. Specific binding of IgE to natural CD23 (low affinity IgE receptor) was inhibited by purified recombinant 37 kDa CD23. Moreover, purified recombinant 37kDa CD23 and interleukin-1 promoted the survival of germinal centre B cells.
000088450 6531_ $$aapoptosis
000088450 6531_ $$abaculovirus
000088450 6531_ $$aCD23 (Fc epsilon RII)
000088450 6531_ $$aIgE
000088450 6531_ $$aFc receptor
000088450 6531_ $$aimmunoglobulin e
000088450 6531_ $$arecombinant dna
000088450 6531_ $$aapoptosis
000088450 6531_ $$ab lymphocyte
000088450 6531_ $$ainsect
000088450 700__ $$aGraber, P.
000088450 700__ $$aJansen, K. U.
000088450 700__ $$aPochon, S.
000088450 700__ $$aShields, J.
000088450 700__ $$aAubonney, N.
000088450 700__ $$0240363$$aTurcatti, G.$$g103994
000088450 700__ $$aBonnefoy, J. Y.
000088450 773__ $$j149$$k2$$q215-226$$tJournal of Immunological Methods
000088450 909C0 $$0252118$$pPTCB$$xU11378
000088450 909CO $$ooai:infoscience.tind.io:88450$$pSV$$particle
000088450 937__ $$aEPFL-ARTICLE-88450
000088450 970__ $$a18/BSF
000088450 973__ $$aOTHER$$sPUBLISHED
000088450 980__ $$aARTICLE